Lipid and protein changes in jojoba callus under salt stress

Abstract

Calluses of jojoba [Simmondsia chinensis (Link) Schneider] were grown for two months in medium either without salt (control), or with 150 mM NaCl (treated). During the first month, the salt-treated calluses grew much more slowly than the controls, and most of them (60%) became necrotic. Afterwards, the salt-resistant calluses grew faster for one more month. Their morphological aspect was similar to that of the controls but their cells appeared enlarged and with thicker walls. Polypeptides of 18, 27 and 49 kDa, which accumulated in salt-treated calluses, appeared as marker proteins for salt adaptation