Genetic control of somatic embryogenesis in alfalfa

Abstract

The genetic control of somatic embryogenesis in alfalfa (Medicago sativa L.) was studied using one nonembryogenic and three embryogenic genotypes: A70-34, a selection from 'Rangelander'; RA3, a selection from 'Regen-S'; and C2-4, a selection from a breeding population that had A70-34 in its pedigree. Crosses of embryogenic × embryogenic and embryogenic × nonembryogenic and S 1 and BC 1 testcrosses were evaluated for in vitro regeneration. Selfing reduced the expression of the trait. Somatic embryogenesis was dominant and explained by two loci. All three regenerating genotypes shared a common genetic system.Key words: alfalfa, Medicago sativa, somatic embryogenesis, tissue culture.