Cloning, mapping and molecular analysis of the pyrG (orotidine-5'-phosphate decarboxylase) gene of Aspergillus nidulans

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.1K citations

Genetics, Puc19 Vectors, Bacteriophage +9

Studies on transformation of Escherichia coli with plasmids

Douglas Hanahan · Journal of Molecular Biology · 1983 · 11.2K citations

Bacteriology, Biotechnology, Escherichia Coli +4

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

Jef D. Boeke, Francois La Croute, Gerald R. Fink · Molecular and General Genetics MGG · 1984 · 2.4K citations

Orotidine-5′-phosphate Decarboxylase Activity, Cellular Enzymology, Biochemistry +7

A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome

Louise Clarke, John Carbon · Cell · 1976 · 1.3K citations

Entire E. Coli, Colony Bank, Molecular Biology +6