Size‐Controlled Formation of a Calcium Phosphate‐Based Organic–Inorganic Hybrid Vector for Gene Delivery Using Poly(ethylene glycol)‐<b><i>block</i></b>‐poly(aspartic acid)

Abstract

Poly(ethylene glycol) (PEG)‐covered hybrid particles (see Figure) of calcium phosphate (CaP), and plasmid DNA (pDNA) have been prepared in a size‐controllable manner in the presence of a block copolymer. The particles show a substantial colloidal stability as well as tolerance of entrapped pDNA against nuclease attack in the medium, and achieve a significantly higher gene expression compared to conventional precipitates.