Application Possibilities of a Novel Immobilized Glucoamylase

Abstract

Abstract A novel immobilized glucoamylase has been developed, which is suited to act in some new processes, e. g. if the saccharification has to be stopped at a certain DE, if limited reactor capacity makes a short residence time necessary, or if the enzyme is unwanted in the product. Application of this enzyme in complete saccharification of starch has been investigated. Max. obtainable DX at 30% DS has been around 94% with this enzyme, where with the same substrate a DX of 96.0 – 96.5% has been obtained with AMG. In addition to this lower degree of hydrolysis, a process with this enzyme has appeared more dependent on the DE of the substrate. The necessary prefiltrations and the problem of retrogradation have been the limiting factors for using low DE maltodextrins. Potential applications of this enzyme which could have economical interest today include treatment of syrup for adjustment or control of carbohydrate spectra, e. g. for production of high conversion syrups or for reduction of oligosaccharide content in raffinate syrups.