Synthesis and characterisation of zein–curcumin colloidal particles

TLDR

Zein–curcumin composite colloidal particles were synthesized using an antisolvent precipitation method. The particles, 100–150 nm in size and spherical, achieved 1.6–4.1 % curcumin loading with 71–87 % encapsulation efficiency, were amorphous, exhibited enhanced photostability, maintained colloidal stability across physiological pH, and showed strong mucoadhesion with over 60 % curcumin retention for 150 min, confirmed by mucin association on Caco‑2 cells.

Abstract

Zein–curcumin composite colloidal particles were synthesized using an antisolvent precipitation method. The average particle size could be controlled down to 100–150 nm, depending on the solvent system and the ratio of zein and curcumin used in the synthesis. In all cases, spherical particles were obtained, as confirmed by transmission electron microscopy. Depending on the preparation conditions, curcumin load and encapsulation efficiency varied from 1.6 to 4.1% and 71.1 to 86.8%, respectively. Solid state characterization by differential scanning calorimetry and X-ray diffraction indicated the amorphous nature of entrapped curcumin. An UV irradiation study confirmed enhanced photostability of curcumin due to the entrapment of curcumin in the biopolymeric matrix. The particles were also found to have good colloidal stability at a broad range of physiologically relevant pH (1.2, 4.5, 6.7 and 7.4) and in simulated gastro-intestinal conditions. Results from an in vitro mucoadhesion study showed retention of more than 60% curcumin for 150 minutes. The mucoadhesion property was further confirmed from a mucin association study carried out on Caco-2 cells.