A method for isolation of anaerobic bacteria from endodontic specimens

Abstract

abstract – A method for the isolation of anaerobic bacteria from endodontic specimens under continuous anaerobiosis is described. A mobile anaerobe unit supplies a gentle stream of oxygen‐free gas over the tooth when the bacteriologic sample is taken. The paper‐points are then, under oxygen‐free gas, immediately transferred to prereduced, anaerobically sterilized roll tubes. The different isolated anaerobic bacteria are typed with biochemical tests, antibiotic susceptibility testing and gaschromatographic analysis of volatile fatty acids and alcohols.