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Regulation of Reactive Oxygen Species by Nerve Growth Factor but not Bcl-2 as a Novel Mechanism of Protection of PC12 Cells from Superoxide Anion-Induced Death
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1999
Year
Lipid PeroxidationApoptosisCell DeathReactive Oxygen SpeciesRedox BiologyCellular PhysiologyOxidative StressInflammationSuperoxide Anion-induced DeathToxicologySuperoxide DismutaseNeuroimmunologyCell SignalingNeuropharmacologyNeuroprotectionReactive Oxygen SpeciePharmacologyCell BiologyPhysiologyNerve Growth FactorWound HealingMedicine
Although neurotrophins protect PC12 cells and neurons from oxidative stress-induced death, the molecular mechanism of this effect is largely unknown. Xanthine (XA)+xanthine oxidase (XO) increased the production of the superoxide anion (O2-) and hydrogen peroxide (H2O2), and the death of PC12 cells. Catalase but not superoxide dismutase (SOD) nor a NO scavenger protected PC12 cells from death, indicating that H2O2 is the main effector responsible for this cell death. Both nerve growth factor (NGF) and Bcl-2 protected PC12 cells from O2--induced toxicity. NGF enhanced the production of O2- and suppressed that of H2O2, suggesting that it inhibits the conversion of O2- to H2O2, while Bcl-2 had no such effect. These results suggested that NGF protected the cells from oxidative stress by altering the composition of the reactive oxygen species (ROS) without affecting their total level.