The peptidoglycan hydrolase TcpG is required for efficient conjugative transfer of pCW3 in Clostridium perfringens

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice

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Life Sciences, Bioinformatics, Sequence Analysis +14

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations

Life Sciences, Biotechnology, Molecular Biology +10

A versatile quick-prep of genomic DNA from Gram-positive bacteria

Andreas Pospiech · Trends in Genetics · 1995 · 737 citations

Life Sciences, Microbiology, Molecular Biology +10

Evolutionary history, structural features and biochemical diversity of the NlpC/P60 superfamily of enzymes

Vivek Anantharaman, L. Aravind · Genome biology · 2003 · 375 citations · Full text

Life Sciences, Biochemistry, Microbiology +12

Molecular cloning and nucleotide sequence of the gene encoding the major peptidoglycan hydrolase of Lactococcus lactis, a muramidase needed for cell separation

Girbe Buist, Jan Kok, Kees Leenhouts et al. · Journal of Bacteriology · 1995 · 247 citations · Full text

Life Sciences, Microbiology, Molecular Biology +13