Do Stable Isotopes Reflect the Food Web Development in Regenerating Ecosystems?

Abstract

We evaluated the use of delta15N- and delta13C-values to monitor the development of food web complexity and biodiversity in a regenerating ecosystem. Therefore a model food chain was established feeding cultivated woodlice (Porcellio dilatatus) on a cellulolytic fungus (Chaetomium globosum) grown on cellulose paper. Two diets of different quality (C:N ratios of 54 vs. 200) with different delta15N- (1.3% vs. 3.1%) but identical delta13C-values caused low and high dietary stress in animals of treatment A and B, respectively. After an incubation time of 7 weeks amount, elemental and isotopic composition of collected faeces and exuviae as well as woodlice and remaining food were determined. The increase of delta15N-values of woodlice relative to the diet was 5.7% and 2.5% in treatments A and B, respectively, whereas delta13C-shifts were 1.0% and 1.6%, showing a reverse relationship. Modelling of elemental and isotopic mass balances indicated that faeces recycling explains the unexpected high 15N-enrichments. Moreover, 13C-enrichments were positively correlated to the degree of starvation. Considering the effects of starvation and recycling of faeces, stable isotopes represent a useful tool to elucidate trophic interactions in regenerating food webs.