Characterization of a “silencer” in yeast: A DNA sequence with properties opposite to those of a transcriptional enhancer

Detection of specific sequences among DNA fragments separated by gel electrophoresis

E.M. Southern · Journal of Molecular Biology · 1975 · 32.8K citations

Life Sciences, Molecular Biology, Molecular Genetics +12

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

Peter Rigby, Marianne Dieckmann, Carl Rhodes et al. · Journal of Molecular Biology · 1977 · 11.7K citations

Life Sciences, Molecular Biology, Molecular Genetics +14

Studies on transformation of Escherichia coli with plasmids

Douglas Hanahan · Journal of Molecular Biology · 1983 · 11.3K citations

Life Sciences, Genetics, Microbiology +12

A rapid boiling method for the preparation of bacterial plasmids

David S. Holmes, Michael E. Quigley · Analytical Biochemistry · 1981 · 3.3K citations

Life Sciences, Microbiology, Molecular Biology +13

[12] One-step gene disruption in yeast

Rodney Rothstein · Methods in enzymology on CD-ROM/Methods in enzymology · 1983 · 3.3K citations

Life Sciences, Genetics, Molecular Genetics +7