ASSOCIATION OF TYROSINE HYDROXYLASE WITH SYNAPTIC VESICLES IN BOVINE CAUDATE NUCLEUS¹

Abstract

Abstract— The subcellular distribution of tyrosine hydroxylase in the bovine caudate nucleus was investigated, with five other enzymes serving as biochemical markers, and coordinated with electron microscopic observations. Tyrosine hydroxylase is particulate bound and has relatively high specific activities in the synaptosome‐enriched subfractions and in the synaptic vesicle fraction. The association of tyrosine hydroxylase with the synaptic vesicles is consistent with the theory of feedback regulation of catecholamine synthesis.