ENERGETICS OF DNA REPAIR IN UV‐IRRADIATED PERIPHERAL BLOOD LEUKOCYTES FROM CHRONIC MYELOID LEUKAEMIA PATIENTS

Abstract

Abstract— The relationship between energy metabolism and UV‐induced (251 nm) DNA repair has been investigated in leukocytes obtained from peripheral blood of chronic myeloid leukaemia patients. Energy supply was modulated by using inhibitors of respiratory (antimvcin‐A) and glycolytic (2‐deoxy‐o‐glucose and other glucose analogues) pathways for adenosine‐triphosphatc (ATP) production. DNA repair was measured by the unscheduled DNA synthesis technique. Parameters of energy metabolism like glucose utilization. lactate production and ATP content of cells. were measured under similar experimental conditions. The observations made indicate the following results: (1) ATP for DNA repair can be supplied by the respiratory and/or by the glycolytic pathway. (2) In the absence of respiration, the rate of glycolysis bears a linear correlation with DNA repair. (3) A minimum threshold rate of ATP‐production is necessary for DNA repair.