Rapid and high-density covalent immobilization of Rhizomucor miehei lipase using a multi component reaction: application in biodiesel production

Abstract

Rapid and high capacity immobilization of<italic>Rhizomucor miehei</italic>lipase on aldehyde-functionalized supports was performed under mild condition<italic>via</italic>a multi component reaction. The mechanism of immobilization reaction was determined as the Ugi reaction.