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Direct Analysis and Identification of Triterpene Glycosides by LC/MS in Black Cohosh,<i>Cimicifuga racemosa</i>, and in Several Commercially Available Black Cohosh Products
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2000
Year
GlycobiologyDirect AnalysisBlack CohoshGas ChromatographyBioanalysisAnalytical ChemistryLiquid ChromatographyPhytochemicalChromatographyGlycosylationBiochemistryBioassay-guided IsolationPharmacologyPhytochemistryBlack Cohosh ProductsTriterpene GlycosidesMass SpectrometryMolecular WeightMedicineDrug Analysis
A method to directly identify triterpene glycosides using reversed-phase liquid chromatography with positive atmospheric pressure chemical ionization mass spectrometry (LC/(+)APCIMS) was developed. Based on the analysis of the molecular weight, fragment ions, selected ion chromatograms, a number of triterpene glycosides, including actein, 27-deoxyactein, cimicfugoside M, and cimicifugoside, from Cimicifuga racemosa were studied. A chromone, cimifugin, from C. foetida was also identified. Cimicifugoside M and cimifugin can specifically serve as indicators for species identification. The method can, therefore, be used to distinguish black cohosh products from among different plant species for quality control purposes.