Click Chemistry as an Efficient Method for Preparing a Sensitive DNA Probe for Photochemical Ligation

Abstract

Speed of light: We report a new photosensitive probe that takes advantage of the electronic structural changes associated with the formation of triazole rings in CuI-catalyzed Huisgen 1,3-dipolar cycloadditions between organic azides and terminal alkynes. We have succeeded in performing photoligation on a timescale of seconds. We also report an efficient procedure using photochemical ligation for the preparation of a DNA probe for quick SNP typing.