Purification and characterization of heterologously expressed nitrilases from filamentous fungi

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

Mark A. Bradford · Analytical Biochemistry · 1976 · 225.4K citations

Life Sciences, Biochemistry, Protein Biochemistry +7

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Marion M. Bradford · Analytical Biochemistry · 1976 · 211.2K citations

Life Sciences, Biochemistry, Protein Biochemistry +7

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice

Julie Thompson, Desmond G. Higgins, Toby J. Gibson · Nucleic Acids Research · 1994 · 64.9K citations · Full text

Life Sciences, Bioinformatics, Sequence Analysis +14

Overexpression of Trigger Factor Prevents Aggregation of Recombinant Proteins in <i>Escherichia coli</i>

Kazuyo Nishihara, Masaaki Kanemori, Hideki Yanagi et al. · Applied and Environmental Microbiology · 2000 · 305 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +17