Electrically Addressed Fabrication of Aptamer‐Based Array Electrodes

TLDR

The method optimizes applied potential, time, and scan rate to electrically address aptamer immobilization on arrayed gold electrodes. The electrically addressed approach successfully immobilizes human IgE aptamers on arrayed gold electrodes, yielding specific IgE recognition and offering rapid, simple, and automated fabrication.

Abstract

Abstract An aptamer immobilization method based electrically addressed fabrication has been developed for the preparation of aptamer‐modified arrayed electrodes, by which the human IgE aptamer was oriented and immobilized on the gold electrode surface. The optimization of the experimental conditions including the applied potential, time and scan rate of potential was investigated. The method was successfully used to immobilize the aptamer onto the desired electrodes, pixel by pixel, based on the electrically addressed approach. Compared to the control electrodes, the resulting aptamer‐modified electrodes showed their specific recognition for human IgE. The present method owns several advantages such as rapid and simple immobilization as well as its automatic addressed capability by the electric approach.