An Accurate Method for Cholesterol Analysis in Bile

Abstract

Abstract An accurate method for quantitative analysis of cholesterol in human bile is presented. Bile cholesterol was extracted in a single solvent (chloroform) in single step and 1H NMR spectra of the extract were obtained by suppressing chloroform signal by presaturation. Cholesterol was quantitatively estimated through integral area of its H‐18 methyl signal which invariably appears distinctly in the nuclear magnetic resonance (NMR) spectrum. Precision and accuracy were determined from the recovery of added cholesterol from bile. Excellent correlation was observed between added and recovered cholesterol (R2=1). Direct and accurate analysis of cholesterol following simple extraction method as presented here may have important implications.