Photochemistry and Photobiology · 1981 · 48 citations · 21 references
Natural SciencesBiochemistryBiophysicsBiophysical ChemistryMolecular BiophysicsSpectroscopyBacterial PhysiologyBiophysical KineticsDecay ProcessesFluorescence SpectroscopySpectroscopy Of BiomoleculesAbsorption SpectraDecay Time
Abstract— The decay time course of intermediate M of bacteriorhodopsin was investigated by flash spectrophotometry. The decay was composed of two exponentials showing the existence of two forms of intermediate absorbing around 410 nm. The two were very different in kinetic character whereas the absorption spectra were almost the same. The relative yield of the two components was a function of the intensity of the exciting flash and the slower component disappeared when the flash intensity was made very small. A model based on the trimeric cluster structure of bacteriorhodopsin is proposed.
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