Determination of Plasma Acetoacetate and D-β-Hydroxy butyrate in New-born Infants by an Enzymatic Fluorometric Micro-method

Abstract

AbstractA simple accurate method using 0.1 ml of plasma for determination of acetoacetate (AcAc) and D-β-hydroxybutarate (BHB) is described and applied to new-born infants. Umbilical cord BHB paralleled maternal values. BHB and AcAc increased in the first days of life and decreased after intravenous glucose. Capillary and venous values were well correlated.Key Words: AcetoacetateD-β-Hydroxybutyrateenzymatic fluorometric micro-methodnew-born infants