Kinetic Spectrophotometric Determination of Thiols and Ascorbic Acid

Abstract

Abstract This paper describes a kinetic spectrophotometric method for the determination of L‐ascorbic acid (AA) and thiols (RSH). Absorbance of Fe(II)‐phen complex formed during the reaction of AA or RSH with Fe(III)‐phen was continuously measured at 510 nm by double‐beam spectrophotometer with flow cell. For determination some thiols, the catalytic effect of Cu2+ ions was used. AA and RSH can be determined in concentration ranges from 4.0×10−6 to 4.0×10−5 M and from 8.0×10−6 to 8.0×10−5 M, respectively. The applicability of the proposed method was demonstrated by determination of chosen compounds in pharmaceutical dosage forms. Keywords: Kinetic determinationspectrophotometrythiolsascorbic acid