Journal of Clinical Microbiology · 2011 · 38 citations · 19 references
Life SciencesInfectious DiseasesMicrobiologyDiagnostic MicrobiologyMedical MicrobiologyMolecular Diagnostics16S Ribosomal Ribonucleic Acid AnalysisInfectious Disease Molecular TestingPathogen DetectionPolymerase Chain ReactionBacterial Load QuantificationKappa CoefficientKappa StatisticPcr-based DetectionPolymerase Chain Reaction (Pcr) AssaysPolymerase Chain Reaction (Pcr) DiagnosticsQuantitative Pcr DetectionReal-time Polymerase Chain Reaction (Pcr)
Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399).
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