Heparin cofactor II (RbHCII) from rock bream (Oplegnathus fasciatus): Molecular characterization, cloning and expression analysis

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

Mark A. Bradford · Analytical Biochemistry · 1976 · 225.4K citations

Life Sciences, Biochemistry, Protein Biochemistry +7

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Kenneth J. Livak, Thomas D. Schmittgen · Methods · 2001 · 181.5K citations

Life Sciences, Molecular Biology, Molecular Genetics +9

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice

Julie Thompson, Desmond G. Higgins, Toby J. Gibson · Nucleic Acids Research · 1994 · 64.9K citations · Full text

Life Sciences, Bioinformatics, Sequence Analysis +14

MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment

Sudhir Kumar · Briefings in Bioinformatics · 2004 · 11.8K citations · Full text

Life Sciences, Bioinformatics, Evolutionary Biology +16

The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling

Konstantin Arnold, Lorenza Bordoli, Jürgen Kopp et al. · Bioinformatics · 2005 · 7.1K citations · Full text

Life Sciences, Biochemistry, Bioinformatics +12