FEMS Microbiology Letters · 1994 · 89 citations · 16 references
Life SciencesMicrobiologyVeterinary MicrobiologyMicrobial GeneticsMolecular EpidemiologyPoultry MicrobiologyBacterial EpidemiologyFoodborne PathogensMolecular TypingPathogen DetectionPlasmid BiologyPlasmid GeneticsDna Hybridization AssaysEpidemiological DiagnosisGel ElectrophoresisMolecular Typing Of PathogensPlasmid InheritancePlasmid StructurePulsed-field Gel ElectrophoresisPulsed-field Gel Electrophoresis Typing
Using pulsed-field gel electrophoresis (PFGE), nine profile types were identified in XbaI digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to pulsed-field profile (PFP) 1 predominated. DNA-DNA hybridization studies demonstrated that a band of approximately 57 kb was associated with the presence of the 38 MDa S. enteritidis 'serovar-specific' plasmid. It is concluded that PFGE provides a method for discriminating strains of S. enteritidis PT 4 suitable for epidemiological investigations.
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A phage-typing scheme for<i>Salmonella enteritidis</i>
L. R. Ward, B. Rowe · Epidemiology and Infection · 1987 · 464 citations · Full text