FEBS Letters · 2004 · 36 citations · 21 references
Life SciencesGeneticsMolecular BiologyGene ExpressionTranscriptionTranscriptional RegulationPromoter RegulationRna Polymerase I TranscriptionTranscription ElongationTranscription FactorsTranscription In EukaryotesTranscription InitiationRna Polymerase I ActivityRna Polymerase I FunctionRna Polymerase I Promoter RegulationRna Polymerase I RecruitmentRna Polymerase I RegulationRna Polymerase I Transcription FactorsRna Polymerase InitiationTranscription Initiation By Rna Polymerase I
The amounts of RNA polymerase I (Pol I) and basal rDNA transcription factors were determined in yeast whole cell extracts. A 17-fold excess of Pol I was found compared to the Pol I-specific initiation factors upstream activating factor (UAF) and core factor (CF) which underlines that both initiation factors interact with a minor fraction of Pol I when rDNA transcription is active. Surprisingly, Rrn3p, another Pol I-specific initiation factor, is more abundant in cell lysates than UAF and CF. Our analyses revealed that a large fraction of cellular Rrn3p is not associated with Pol I. However, the amount of initiation-active Rrn3p which forms a stable complex with Pol I corresponds to the levels of UAF and CF which have been shown to bind the promoter. Initiation-active Rrn3p dissociates from the template during or immediately after Pol I has switched from initiation to elongation. Our data support a model in which the elongating Pol I leaves the initiation factors UAF, CF and Rrn3p close by the promoter.
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Global analysis of protein expression in yeast
Sina Ghaemmaghami, Won‐Ki Huh, Kiowa Bower et al. · Nature · 2003 · 3.8K citations
Chromatin structures and transcription of rDNA in yeast<i>Saccharomyces cerevisiae</i>
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Jonathan A. Claypool, Sarah L. French, Katsuki Johzuka et al. · Molecular Biology of the Cell · 2003 · 173 citations · Full text