International Journal of Immunogenetics · 1991 · 29 citations · 15 references
Life SciencesGeneticsMolecular GeneticsHuman GeneticsImmunogeneticsMedical GeneticsAllele NomenclatureHla TypingMajor Histocompatibility ComplexMolecular TypingPolymerase Chain ReactionRestriction Enzyme TechnologyGenomic Dna AnalysisHla Allele AssignmentHla Allele PolymorphismHla Risk AllelesMolecular Hla TypingOligonucleotide HybridizationPolymerase Chain Reaction Hla TypingRestriction Fragment Length Polymorphism Analysis
Polymerase chain reaction (PCR) amplification of the second exon of DRB genes from genomic DNA is described. Sequence-specific oligonucleotide (SSO) probes have been designed to allow for the typing of DRB alleles. The methods are described in detail and the results of a trial on both homozygous typing cells and restriction fragment length polymorphism (RFLP)-typed local caucasoid controls are given. The ease of use of this form of DRB typing is emphasized and potential complications are discussed.
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Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Randall K. Saiki, David H. Gelfand, Susanne Stoffel et al. · Science · 1988 · 17.2K citations
William I. Wood, Jane Gitschier, L A Lasky et al. · Proceedings of the National Academy of Sciences · 1985 · 810 citations · Full text