Genome Manipulation through Site-Specific Recombination

Abstract

Abstract Several DNA site-specific recombination systems have been shown to function in higher eukaryotic cells. These two-component systems consist of a single-polypeptide recombinase and a short recognition sequence of less than 35 bp. Strategic placement of the recognition sites into the plant genome has permitted the deletion, inversion, integration, and translocation of host and introduced DNA fragments. Recombinase-based strategies afford precise and predictable engineering of the plant genome.