Determination of binary mixtures of sulfonamides by photochemically induced fluorescence using partial least squares multivariate calibration

Abstract

Binary mixtures of sulfamethazine (SMTZ), sulfamerazine (SMRZ) and sulfadiazine (SDZ) were determined by room-temperature photochemically induced fluorescence. The resolution was accomplished by partial least squares (PLS) multivariate calibration. The fluorescence intensity of these heterocyclic sulfonamides was measured after ultraviolet irradiation for 12 min. The photoproducts obtained showed analytically useful fluorescence signals when an excitation wavelength of 284 nm was used. PLS was applied with previous optimization of the calibration matrix by the PLS-1 method. The multivariate factor analysis based calibration method allowed the simultaneous determination of SMTZ–SMRZ, SMTZ–SDZ and SMRZ–SDZ mixtures.