Characteristics of Feeding Behaviors of Myzus persicae (Hemiptera: Aphididae) Depending on Inflow Concentrations of Dinotefuran

Abstract

Dinotefuran을 농도 별로 처리하여 420분 동안 복숭아혹진딧물의 섭식행동의 변화와 식물체로 이행된 살충제의 농도를 측정하였다. 약제처리 후 30분부터는 섭식패턴의 변화를 나타내기 시작하며 구침을 빼는 행동과 구침의 활성이 일어나는 패턴, 체관부를 섭식하는 패턴에 있어 농도별 차이를 보였다. 특히 식물체에 유입된 dinotefuran의 농도가 높아질수록 구침을 빼거나 구침의 활성이 일어나는 약제에 대한 반응시간이 빨리 나타났으며, 구침을 빼고 있는 총 시간이 길게 나타났다. HPLC를 이용하여 잎에 잔류한 dinotefuran의 농도를 측정한 결과, 추천농도인 100 mg/L로 2시간 동안 처리시 5.24<TEX>${\sim}$</TEX>7.24 mg/L로 잔류하였으며, 복숭아혹진딧물은 약제처리 후 약 60분 경과 후부터 살충제를 감지하고 약제에 대한 반응을 개시하는 것을 확인하였다. The changes of feeding pattern and the amount of insecticide penetrated into the leaf were monitored for 420 min after treatment of three concentrations of dinotefuran. At about 30 min after treatment of insecticide, the overall feeding pattern in EPG (Electrical Penetration Graph) monitoring was started to change, and there was significant difference in proportions of NP (non-penetration), PA (pathway activity), and phloem-feeding patterns among the different concentrations of dinotefuran treatment. Especially, as the amount of insecticide penetrated into a leaf increased, the reactive behaviors against this insecticide such as withdrawal of proboscis and movements of stylet of aphid were more quickly exhibited. And also, total time at which the proboscis of the aphids did not penetrate the plant was getting longer. The amount of dinotefuran penetrated into a leaf was monitored with HPLC. When the recommended concentration (100 mg/L) of the insecticide was treated, 5.24<TEX>${\sim}$</TEX>7.24 mg/L of the insecticide was detected from the leaf, and the proboscis of the aphids was apparently withdrawn from the leaf at approximately 60 minutes after treatment of this insecticide.