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Electrospray Mass Spectrometry: Complexation Between 1-Anilinonaphthalene-8-sulphonate and Proteins
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1996
Year
Protein ChemistryElectrospray Mass SpectrometryHydrophobic Fluorescent DyeBiochemistryProtein FoldingApparent AgreementBioanalysisMass SpectrometryBiological Mass SpectrometryMolecular BiologyNatural SciencesProtein Mass SpectrometryNative Mass SpectrometryAnalytical ChemistryMedicineSingle-molecule DetectionBiomolecular Analysis
Complexation of the hydrophobic fluorescent dye 1-anilinonaphthalene-8-sulphonate with a number of proteins was examined by electrospray ionization mass spectrometry (ESMS). The apparent agreement between the present data and those obtained by fluorescence spectroscopy underlines the role of ESMS in the overall approach to protein folding and unfolding.