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VARIATIONS IN EXPRESSION OF CELL MEMBRANE ANTIGENS BY CULTURED CELLS
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1973
Year
Immunocytochemical TechniqueBiochemistryBioanalysisImmunologyMean Cell VolumeImmunophenotypingCell CultureAutoimmunityAntigen ProcessingImmunoglobulin EImmunotherapyMedicineCell BiologyCellular PhysiologyAlg IncreaseCell Specialization
The binding of fluorescein isothiocyanate-labeled antilymphocytie globulin (ALG) to the membranes of cells from human lymphoid cell cultures has been studied. Broad variations both on different lines and within the same line have been found. An inverse relationship was observed between cell growth and the percentage of labeled cells. A decrease of the percentage of labeled cells was noticed as the mean cell volume increased. To verify a cyclic dependency of the above phenomena a cell culture has been synchronized. The labeling index decreased during S and G2 phases and increased during G1. Furthermore, it has been shown that membrane antigens detected by ALG increase from the beginning of a cell culture until its establishment as a cell line.