Science · 1996 · 264 citations · 21 references
Life SciencesCell BiologyMolecular BiologyEndocytosisIntracellular TransportMembrane TraffickingEndomembrane SystemEndosomal TraffickingIntracellular TraffickingLate EndosomesReceptor-mediated EndocytosisVesicle TraffickingActin-based Cell MotilityActin-based MotilityActin-membrane CouplingEndocytosis PathwaysMembrane TrafficMyosin MotorsMyosin RegulationYeast Endocytosis
Type I myosins are thought to drive actin-dependent membrane motility, but the direct demonstration in vivo of their involvement in specific cellular processes has been difficult. Deletion of the genes MYO3 and MYO5, which encode the yeast type I myosins, almost abolished growth. A double-deleted mutant complemented with a MYO5 temperature-sensitive allele (myo5-1) showed a strong defect in the internalization step of receptor-mediated endocytosis, whereas the secretory pathway remained apparently unaffected. Thus, myosin I activity is required for a budding event in endocytosis but not for several other aspects of membrane traffic.
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