Spectrophotometric Determination of Some Antibacterial Drugs Using p-Nitrophenol

Abstract

A simple, quick and sensitive spectrophotometric method for pipemidic acid, norfloxacin and ciprofloxacin lactate is described, based on a reaction with p-nitrophenol in water medium, apparently by a charge-transfer mechanism, to yield 1:1 complexes with analytically useful maximum absorption at 404 nm, 407nm and 403nm. Optimum conditions for determination, linear calibration range and apparent molar absorptivities have been reported. The methods are highly accurate and have been successfully applied to the determination of pipemidic acid, norfloxacin and ciprofloxacin lactate in tablets. The results are in good agreement with the official methods.