Facile Quantification of Enantiomeric Excess <i>and</i> Concentration with Indicator-Displacement Assays: An Example in the Analyses of α-Hydroxyacids

Abstract

An enantioselective indicator-displacement assay was developed to determine enantiomeric excess (ee) and concentration of α-hydroxyacid samples with two independent absorption measurements. The inherent relationship between the absorbance of the indicator-displacement ensemble and the overall concentration and ee of the analyte was established through solution equilibria.