Molecular Ecology Notes · 2004 · 10 citations · 7 references
Life SciencesGeneticsMolecular GeneticsForest GeneticsPlant GeneticsPopulation GeneticsChloroplast GeneticsGene MappingMolecular MarkersMolecular Markers In PlantsQuantitative Trait LociQuantitative Trait Locus MappingChloroplast Dna MarkersChloroplast OriginMicrosatellite AnalysisMicrosatellite Marker AnalysisMicrosatellite MarkersMicrosatellite VariationObserved HeterozygosityPaternal Inheritance Patterns
Abstract Five polymorphic microsatellite markers are described for coast redwood, a hexaploid conifer. Dinucleotide (three), trinucleotide (one) and tetranucleotide (one) repeat loci were isolated from a genomic plasmid library and amplified in a test population of 25 second‐growth redwoods. There were three to 11 alleles per locus and observed heterozygosities ranged from 0.00 to 0.80. The nonheterozygous locus (a CTTA repeat) is probably of chloroplast origin and may have potential as a marker for paternal inheritance.
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High-Throughput DNA Extraction Method Suitable for PCR
Zhanguo Xin, Jeff Velten, Melvin J. Oliver et al. · BioTechniques · 2003 · 217 citations · Full text