Light‐Induced Protein Dimerization by One‐ and Two‐Photon Activation of Gibberellic Acid Derivatives in Living Cells

Abstract

We developed a highly efficient system for light-induced protein dimerization in live cells using photo-caged derivatives of the phytohormone gibberellic acid (GA3 ). We demonstrate the application of the photo-activatable chemical inducer of dimerization (CID) for the control of protein translocation with high spatiotemporal precision using light as an external trigger. Furthermore, we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.