Melanie II – a third‐generation software package for analysis of two‐dimensional electrophoresis images: II. Algorithms

TLDR

Third‑generation 2‑DE image analysis software now combines advanced GUIs with comprehensive spot data analysis, and most tools are built on algorithms from image processing, vision, AI, and machine learning. This article introduces the main algorithms implemented in the Melanie II 2‑D PAGE software package. These algorithms are applied as program features, with their applications detailed in a companion article (Appel et al., Electrophoresis 1997).

Abstract

Abstract After two generations of software systems for the analysis of two‐dimensional electrophoresis (2‐DE) images, a third generation of such software packages has recently emerged that combines state‐of‐the‐art graphical user interfaces with comprehensive spot data analysis capabilities. A key characteristic common to most of these software packages is that many of their tools are implementations of algorithms that resulted from research areas such as image processing, vision, artifical intelligence or machine learning. This article presents the main algorithms implemented in the Melanie II 2‐D PAGE software package. The applications of these algorithms, embodied as the feature of the program, are explained in an accompanying article (R. D. Appel et al.; Electrophoresis 1997, 18 , 2724–2734).