An improved microspectrofluorimeter with automatic digital data logging: Construction and operation

Abstract

SUMMARY This paper describes an instrument for microspectrofluorimetry, based on the Leitz microspectrograph. Important features are monochromatic epi‐illumination, a reference channel and a measuring system using photon counting with automatic recording of data in digital form. To minimize fading, measurements of fluorescence intensity can be made within as little as 200 msec from the commencement of irradiation.