Detection of papillomavirus DNA in oral papillomas and carcinomas: application of <i>in situ</i> hybridization with biotinylated HPV 16 probes

Abstract

Four oral papillomas and 7 carcinomas were studied for the presence of HPV DNA by means of in situ hybridization. Hybridization was carried out with a HPV 16 probe labelled with biotin under different conditions (nick translation/photobiotin). Subsequently, a modified biotin-avidin-alkaline phosphatase procedure was used to visualize virus infected cells. Four/4 papillomas and 4/7 carcinomas were seen to contain HPV harbouring cells. Positive cells were located at intermediate and superficial cell layers in papillomas and in keratinized zones in carcinomas. Analogous results were found with nick translated and photobiotinylated probes. DNA-biotinylation in conjunction with the biotin-avidin-alkaline phosphatase detection system proved to be a sensitive, convenient and rapid modification of in situ hybridization.