3‘-Immobilized Probes with 2‘-Caps:  Synthesis of Oligonucleotides with 2‘-<i>N</i>-Methyl-2‘-(anthraquinone carboxamido)uridine Residues

Abstract

[reaction: see text] A synthesis for oligodeoxynucleotides with a 3'-terminal 2'-N-methyl-2'-acylamido-2'-deoxyuridine residue was developed. Unlike their unmethylated counterparts, these oligodeoxynucleotides can be stably immobilized on aldehyde-displaying glass surfaces to provide DNA microarrays. An anthraquinone carboxamido group as a 2'-substituent doubled the capture efficiency of an immobilized tetradecamer.