Proceedings of the National Academy of Sciences · 1971 · 38 citations · 13 references
Life SciencesBiochemistryMolecular BiologyProtein BiochemistryProtein TranslationProtein-protein InteractionsProtein Complex AssemblyRibosome FunctionStable Protein ComplexesTranslation FactorsTranslation In ProkaryotesTranslation InitiationBacterial Ribosome FunctionEscherichia ColiEukaryotic Initiation FactorsEukaryotic Ribosome FunctionEukaryotic Translation Initiation FactorsProkaryotic Initiation FactorsProkaryotic Translation Initiation FactorsTranslation Initiation Complexes
Initiation factor FII (F(3), factor B) has been purified from Escherichia coli Q13 to apparent homogeneity. It is a heat-stable basic protein, consisting of a single polypeptide chain of molecular weight 21,000. FII is required, in addition to the other factors FI and FIII, for the formation of a 70S complex containing fMet-tRNA, poly(U,G) (used as a model messenger RNA), and the ribosome. FII also is capable of dissociating 70S ribosomes to 30S and 50S subunits. Evidence is presented that both activities are mediated by the same protein.
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Translation of the genetic message
Kentaro Iwasaki, Steven L. Sabol, Albert J. Wahba et al. · Archives of Biochemistry and Biophysics · 1968 · 217 citations