A Liquid Chromatography/Tandem Mass Spectrometry Method for Determination of 25-Hydroxy Vitamin D₂ and 25-Hydroxy Vitamin D₃ in Dried Blood Spots: A Potential Adjunct to Diabetes and Cardiometabolic Risk Screening

Abstract

This blood spot assay for 25(OH)D(2) and 25(OH)D(3) provides a convenient and cost-effective alternative to serum assays and can be automated. This may be valuable in large-scale screening for risk of type 1 diabetes, for cardiometabolic risk screening, and for monitoring vitamin D supplementation.