Synthesis of Multi-Domain Proteins Using Expressed Protein Ligation: Strategies for Segmental Isotopic Labeling of Internal Regions

Synthesis of Proteins by Native Chemical Ligation

Philip E. Dawson, Tom W. Muir, Ian Clark‐Lewis et al. · Science · 1994 · 4.1K citations

Life Sciences, Biochemistry, Molecular Biology +17

Attenuated <i>T</i> ₂ relaxation by mutual cancellation of dipole–dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution

Konstantin Pervushin, Roland Riek, Gerhard Wider et al. · Proceedings of the National Academy of Sciences · 1997 · 2.5K citations · Full text

Natural Sciences, Biochemistry, Physics +14

Direct Measurement of Distances and Angles in Biomolecules by NMR in a Dilute Liquid Crystalline Medium

Nico Tjandra, Ad Bax · Science · 1997 · 1.7K citations

Natural Sciences, Biochemistry, Biophysics +13

Expressed protein ligation: A general method for protein engineering

Tom W. Muir, Dolan Sondhi, Philip A. Cole · Proceedings of the National Academy of Sciences · 1998 · 1.2K citations

Life Sciences, Biochemistry, Molecular Biology +16

Production of cyclic peptides and proteins <i>in vivo</i>

Charles P. Scott, Ernesto Abel‐Santos, Mark J. Wall et al. · Proceedings of the National Academy of Sciences · 1999 · 398 citations · Full text

Pharmacy, Biochemistry, Biotechnology +17