Improving the Insecticidal Activity against Resistant <i>Culex quinquefasciatus</i> Mosquitoes by Expression of Chitinase Gene <i>chiAC</i> in <i>Bacillus sphaericus</i>

Yajun Cai, Jianpin Yan, Xiaomin Hu, Bei Han, Zhiming Yuan

Applied and Environmental Microbiology · 2007 · 28 citations · 14 references

Abstract

ABSTRACT Expression of a chitinase gene, chiAC , from Bacillus thuringiensis in B. sphaericus 2297 using the binary toxin promoter yielded a recombinant strain that was 4,297-fold more toxic than strain 2297 against resistant Culex quinquefasciatus . These results show that this chitinase can synergize the toxicity of the binary toxin against mosquitoes and thus may be useful in managing mosquito resistance to B. sphaericus .

References

14