A Novel Method for Synthesis of Cellooligosaccharide Derivatives by Using Enzyme Catalyst

Abstract

Abstract A new methodology for regio and stereoselective synthesis of cellooligosaccharide derivatives has been developed by utilizing the following two enzymatic reactions: A cellulase-catalyzed stereoselective lactosylation of an alkyl cellobioside giving rise to a new oligosaccharide having a D-galactose unit at the non-reducing end and a β-galactosidase-catalyzed regioselective cleavage of the terminal D-galactose unit of the product.