N-Methyl-d-aspartate (NMDA) Receptor Subunit NR1 Forms the Substrate for Oligomeric Assembly of the NMDA Receptor

Abstract

The time course of the assembly of the N-methyl-d-aspartate receptor was examined in a cell line expressing it under the control of the dexamethasone promoter. These studies suggested a delay between the appearance of the NR1 and NR2A subunits and their stable association as examined by co-immunoprecipitation of NR1 and NR2A. This prompted us the and of the subunits and the studies the NR1 was in a and and and NR1 was and was under of the of and in the was of NR1 was NR1 and and the assembly of the N-methyl-d-aspartate The time course of the assembly of the N-methyl-d-aspartate receptor was examined in a cell line expressing it under the control of the dexamethasone promoter. These studies suggested a delay between the appearance of the NR1 and NR2A subunits and their stable association as examined by co-immunoprecipitation of NR1 and NR2A. This prompted us the and of the subunits and the studies the NR1 was in a and and and NR1 was and was under of the of and in the was of NR1 was NR1 and and the assembly of the N-methyl-d-aspartate The N-methyl-d-aspartate receptor of the receptor of of receptor and The NR1 by a in and The of by in The of and of studies of cell the receptor subunits the NR1 and the of studies the NR1 and subunits the of a The NR1 the and the of the studies the suggested it as a NR1 and studies suggested NR1 in and the of the receptor subunits the and studies a These studies the assembly of the receptor by the association of subunits the of the as the and studies of the of it the receptor a it the receptor it by association of of the of the NR1 and subunits suggested a of the assembly of the receptor the of of the subunits a of the receptor assembly NR1 subunits in as subunits a of a the of the receptor the cell the assembly of the receptor examined the time course of the assembly of the receptor in a cell line expressing it under the control of the dexamethasone promoter. These studies delay between the appearance of the subunits and their association as by co-immunoprecipitation of NR1 and This prompted us the and of the subunits and the of their in the the NR1 and in a and and and and of and stable The of was The NR1 and a the stable association and of the subunits NR2A was a the of NR2A as The was the as The was a and the was by the the of by and the a a by a and the the of The was in and a The was the by and The the a and the by The of the was expressing and and was NR1 the a the in of The a of receptor subunits and by and of and The the was by and the and The was and and a was in the the and by of the and was by of the by of the NR2A the and and of the The was was the and The was and and a was as the NR2A in NR2A was and a of was a of NR2A was the NR2A This was in the The was by the the and a The was and a in The of was by and and in in of the a the NR1 and the of was cell line expressing receptor subunits under the control of the dexamethasone The cell line was in in and in of of and NR2A subunits was by of dexamethasone the The expressing in was by the of the of the cell line was and the the in The by and NR1 as and time by co-immunoprecipitation and as in as and of the and of the of was the and by The by and the was in The the by in the and cell by and the of the the the and as and of a a was of in was of the was in a the subunits the in and in the by was by of and a and of the by as the was in a the receptor subunits in and and as and in was a of and by and in of the time and the the the was of the and as the was the a in a of and as the by under a cell the in in The the and in the of was a of as by and and the was of was and the was The and The and and the a The and and The was a The the The of the the was and as the the receptor subunits the was a as was a and the as of of by in and the of the by The a and the by of the was in of and the of and NR2A and cell the cell of receptor dexamethasone and The of the subunits as as NR2A in the cell by This in the appearance of the NR2A a the subunits time course of and NR2A association was examined by cell a of the by The by the NR2A the and NR2A association was a NR2A the the of NR2A of association the of receptor the of and NR2A subunits in the cell line the of receptor and the The of and NR2A the in as of the the receptor the the NR2A This in expressing the receptor NR2A This co-immunoprecipitation of NR2A of subunits The delay in the association of NR1 and the time and of their the time a stable of NR1 and a examined the of the and subunits in and and of the under the of and and The and in the of under This in in the the in the in a the by a of under it of cell expressing under The in the of the of the of The the of the in the The in the and the of a of the the subunits the was expressing of the and the as a and of cell of This a of and the as a in the as suggested by studies of the of it by the of of expressing NR2A a a of the and the of The of NR2A of NR2A and of and a of the in the of was of of the NR2A their of the NR2A of the the of the in the it the the of in in The of of NR2A and in the of and the as of the cell expressing and and in expressing the receptor subunits in the of of the subunits a under NR2A and NR2A in by the in and the in the of of a the of NR2A of the of the NR1 and a the of the NR2A of the of of it NR1 and in the of and in the the the of the of the of of the receptor subunits was examined in of by control and and the of was the of was as was and NR2A in as of the in the the was and a in a in the NR1 and of expressing the receptor of the subunits in the of was by in and their the of The time and the the subunits in and was in a in and a in the of and The the and in NR2A The in the of the in the of NR2A. The and in the of their by the and The of the subunits was examined in The a a of of the stable in the NR2A and of and of This NR2A and and NR2A. was the of NR2A and of subunits their NR2A the of NR2A was in the of NR1 the of NR2A was in in of NR1 the of the The of NR1 was in the in and of the of the and of the of the of the NR2A and of of and in and the NR2A the NR2A stable a in in of the in and the receptor the and the as the of time The the the of the of the of of NR2A and The the by the of the subunits in the by in the as and and the of the subunits and NR1 stable as by and of NR2A and by of the a of a of of of of the co-immunoprecipitation of of NR2A of the as NR2A and and NR1 of and under the of the of receptor the receptor subunits and a of and the the the and a control The of the The in the and the of the the of NR2A co-immunoprecipitation of the co-immunoprecipitation of NR2A. of the and NR2A co-immunoprecipitation of NR2A the was of by co-immunoprecipitation of in the time course of receptor the of and NR2A in the subunits of and the receptor in of the appearance of the a delay of of delay a of the the subunits and their of it the time the of the subunits the studies the subunits and the delay between the of the of the subunits and their the time a stable of NR1 as a receptor the of the subunits under the the and a NR2A and it the in of in the it as a and the of of the receptor the cell and in the by the of NR1 as a the it NR1 stable in the a of the receptor in a was between NR1 The of the the in the of the of NR2A the of of in and as as in their The the of in the and their The of the and by the it it in and in the between and under the of the of the it the subunits the and NR2A of in the of the receptor subunits a the of stable time NR2A and of and of NR2A the of the was the of NR2A and the subunits in a of NR1 was a of and was stable in the of NR1 by it the of stable the of in the of in the of These the and the the of the and stable NR2A the of the of in the of the a a of the was NR2A and NR2A and The in and in the in This the of the subunits the subunits in the as of in the cell the association by the of the between NR1 and NR1 and as The was examined in of in of the studies the of NR1 in the of subunits it as a the stable assembly of the The NR1 a it in a in as a by of the of NR2A a of of NR1 subunits and studies the of NR1 and association the the of the NR1 by association of association the of NR1 the NR1 in receptor and assembly the of NR1 in the and a of NR1 in the of This of NR1 and the and subunits as of NR1 and NR1 was the and of in a NR1 the was the of the the of NR2A and the of NR2A was the the receptor their and in the of the NR2A and These of receptor assembly and the of the NR1 in the assembly of the it a stable the and of the The N-methyl-d-aspartate receptor of the receptor of of receptor and The NR1 by a in and The of by in The of and of studies of cell the receptor subunits the NR1 and the of studies the NR1 and subunits the of a The NR1 the and the of the studies the suggested it as a NR1 and studies suggested NR1 in and the of the receptor subunits N-methyl-d-aspartate N-methyl-d-aspartate the and studies a These studies the assembly of the receptor by the association of subunits the of the as the and studies of the of it the receptor a it the receptor it by association of of the of the NR1 and subunits suggested a of the assembly of the receptor the of of the subunits a of the receptor assembly NR1 subunits in as subunits a of a the of the receptor the cell the assembly of the receptor examined the time course of the assembly of the receptor in a cell line expressing it under the control of the dexamethasone promoter. These studies delay between the appearance of the subunits and their association as by co-immunoprecipitation of NR1 and This prompted us the and of the subunits and the of their in the the NR1 and in a and and and and of and stable The of was The NR1 and a the stable association and of the subunits NR2A was a the of NR2A as The was the as The was a and the was by the the of by and the a a by a and the the of The was in and a The was the by and The the a and the by The of the was expressing and and was NR1 the a the in of The a of receptor subunits and by and of and The the was by and the and The was and and a was in the the and by of the and was by of the by of the NR2A the and and of the The was was the and The was and and a was as the NR2A in NR2A was and a of was a of NR2A was the NR2A This was in the The was by the the and a The was and a in The of was by and and in in of the a the NR1 and the of was cell line expressing receptor subunits under the control of the dexamethasone The cell line was in in and in of of and NR2A subunits was by of dexamethasone the The expressing in was by the of the of the cell line was and the the in The by and NR1 as and time by co-immunoprecipitation and as in as and of the and of the of was the and by The by and the was in The the by in the and cell by and the of the the the and as and of a a was of in was of the was in a the subunits the in and in the by was by of and a and of the by as the was in a the receptor subunits in and and as and in was a of and by and in of the time and the the the was of the and as the was the a in a of and as the by under a cell the in in The the and in the of was a of as by and and the was of was and the was The and The and and the a The and and The was a The the The of the the was and as the the receptor subunits the was a as was a and the as of of by in and the of the by The a and the by of the was in of and the NR2A was a the of NR2A as The was the as The was a and the was by the the of by and the a a by a and the the of The was in and a The was the by and The the a and the by The of the was expressing and and was NR1 the a the in of The a of receptor subunits and by and of and The the was by and the and The was and and a was in the the and by of the and was by of the by of the NR2A the and and of the The was was the and The was and and a was as the NR2A in NR2A was and a of was a of NR2A was the NR2A This was in the The was by the the and a The was and a in The of was by and and in in of the a the NR1 and the of was The cell line expressing receptor subunits under the control of the dexamethasone The cell line was in in and in of of and NR2A subunits was by of dexamethasone the The expressing in was by the of the of the cell line was and the the in The by and NR1 as and time by co-immunoprecipitation and as in as and of the and of the of was the and by The by and the was in The the by in the and cell by and the of the the the and as and of a a was of in was of the was in a the subunits the in and in the by was by of and a and of the by as the was in a the receptor subunits in and and as and in was a of and by and in of the time and the the the was of the and as the was the a in a of and as the by under a cell the in in The the and in the of was a of as by and and the was of was and the was The and The and and the a The and and The was a The the The of the the was and as the the receptor subunits the was a as was a and the as of of by in and the of the by The a and the by of the was in of and the of and NR2A and cell the cell of receptor dexamethasone and The of the subunits as as NR2A in the cell by This in the appearance of the NR2A a the subunits time course of and NR2A association was examined by cell a of the by The by the NR2A the and NR2A association was a NR2A the the of NR2A of association the of receptor the of and NR2A subunits in the cell line the of receptor and the The of and NR2A the in as of the the receptor the the NR2A This in expressing the receptor NR2A This co-immunoprecipitation of NR2A of subunits The delay in the association of NR1 and the time and of their the time a stable of NR1 and a examined the of the and subunits in and and of the under the of and and The and in the of under This in in the the in the in a the by a of under it the subunits the was expressing of the and the as a and of cell of This a of and the as a in the as suggested by studies of the of it by the of of expressing NR2A a a of the and the of The of NR2A of NR2A and of and a of the in the of was of of the NR2A their of the NR2A of the the of the in the it the the of in in The of of NR2A and in the of and the as of the cell expressing and and in expressing the receptor subunits in the of of the subunits a under NR2A and NR2A in by the in and the in the of of a the of NR2A of the of the NR1 and a the of the NR2A of the of of it NR1 and in the of and in the the the of the of the of of the receptor subunits was examined in of by control and and the of was the of was as was and NR2A in as of the in the the was and a in a in the NR1 and of expressing the receptor of the subunits in the of was by in and their the of The time and the the subunits in and was in a in and a in the of and The the and in NR2A The in the of the in the of NR2A. The and in the of their by the and The of the subunits was examined in The a a of of the stable in the NR2A and of and of This NR2A and and NR2A. was the of NR2A and of subunits their NR2A the of NR2A was in the of NR1 the of NR2A was in in of NR1 the of the The of NR1 was in the in and of the of the and of the of the of the NR2A and of of and in and the NR2A the NR2A stable a in in of the in and the receptor the and the as the of time The the the of the of the of of NR2A and The the by the of the subunits in the by in the as and and the of the subunits and NR1 stable as by and of NR2A and by of the a of a of of of of the co-immunoprecipitation of of NR2A of the as NR2A and and NR1 of and under the of the of receptor the receptor subunits and a of and the the the and a control The of the The in the and the of the the of NR2A co-immunoprecipitation of the co-immunoprecipitation of NR2A. of the and NR2A co-immunoprecipitation of NR2A the was of by co-immunoprecipitation of in of and NR2A and cell the cell of receptor dexamethasone and The of the subunits as as NR2A in the cell by This in the appearance of the NR2A a the subunits The time course of and NR2A association was examined by cell a of the by The by the NR2A the and NR2A association was a NR2A the the of NR2A of association the of receptor the of and NR2A subunits in the cell line the of receptor and the The of and NR2A the in as of the the receptor the the NR2A This in expressing the receptor NR2A This co-immunoprecipitation of NR2A of subunits The delay in the association of NR1 and the time and of their the time a stable of NR1 and a examined the of the and subunits in NR1 and and of the under the of and and The and in the of under This in in the the in the in a the by a of under it the subunits the was expressing of the and the as a and of cell of This a of and the as a in the as suggested by studies of the of it by the of of expressing NR2A a a of the and the of The of NR2A of NR2A and of and a of the in the of was of of the NR2A their of the NR2A of the the of the in the it the the of in in The of of NR2A and in the of and the as of and in the the the of the of the of of the receptor subunits was examined in of by control and and the of was the of was as was and NR2A in as of the in the the was and a in a in the NR1 and and in the of their by the and The of the subunits was examined in The a a of of the stable in the NR2A and of and of This NR2A and and NR2A. was the of NR2A and of subunits their NR2A the of NR2A was in the of NR1 the of NR2A was in in of NR1 the of the The of NR1 was in the in and of the of the and of the of the of the NR2A and of NR1 and and the of the subunits and NR1 stable as by and of NR2A and by of the a of a of of of of the co-immunoprecipitation of of NR2A of the as NR2A and and NR1 of and under the the time course of receptor the of and NR2A in the subunits of and the receptor in of the appearance of the a delay of of delay a of the the subunits and their of it the time the of the subunits the studies the subunits and the delay between the of the of the subunits and their the time a stable of NR1 as a receptor the of the subunits under the the and a NR2A and it the in of in the it as a and the of of the receptor the cell and in the by the of NR1 as a the it NR1 stable in the a of the receptor in a was between NR1 The of the the in the of the of NR2A the of of in and as as in their The the of in the and their The of the and by the it it in and in the between and under the of the of the it the subunits the and NR2A of in the of the receptor subunits a the of stable time NR2A and of and of NR2A the of the was the of NR2A and the subunits in a of NR1 was a of and was stable in the of NR1 by it the of stable the of in the of in the of These the and the the of the and stable NR2A the of the of in the of the a a of the was NR2A and NR2A and The in and in the in This the of the subunits the subunits in the as of in the cell the association by the of the between NR1 and NR1 and as The was examined in of in of the studies the of NR1 in the of subunits it as a the stable assembly of the The NR1 a it in a in as a by of the of NR2A a of of NR1 subunits and studies the of NR1 and association the the of the NR1 by association of association the of NR1 the NR1 in receptor and assembly the of NR1 in the and a of NR1 in the of This of NR1 and the and subunits as of NR1 and NR1 was the and of in a NR1 the was the of the the of NR2A and the of NR2A was the the receptor their and in the of the NR2A and These of receptor assembly and the of the NR1 in the assembly of the it a stable the and of the the time course of receptor the of and NR2A in the subunits of and the receptor in of the appearance of the a delay of of delay a of the the subunits and their of it the time the of the subunits the studies the subunits and the delay between the of the of the subunits and their the time a stable of NR1 as a receptor the of the subunits under the the and a NR2A and it the in of in the it as a and the of of the receptor the cell and in the by the of NR1 as a the it NR1 stable in the a of the receptor in a was between NR1 The of the the in the of the of NR2A the of of in and as as in their The the of in the and their The of the and by the it it in and in the between and under the of the of the it the subunits the and NR2A of in the of the receptor subunits a the of stable time NR2A and of and of NR2A the of the was the of NR2A and the subunits in a of NR1 was a of and was stable in the of NR1 by it the of stable the of in the of in the of These the and the the of the and stable NR2A the of the of in the of the a a of the was NR2A and NR2A and The in and in the in This the of the subunits the subunits in the as of in the cell the association by the of the between NR1 and NR1 and as The was examined in of in of the studies the of NR1 in the of subunits it as a the stable assembly of the The NR1 a it in a in as a by of the of NR2A a of of NR1 subunits and studies the of NR1 and association the the of the NR1 by association of association the of NR1 the NR1 in receptor and assembly the of NR1 in the and a of NR1 in the of This of NR1 and the and subunits as of NR1 and NR1 was the and of in a NR1 the was the of the the of NR2A and the of NR2A was the the receptor their and in the of the NR2A and These of receptor assembly and the of the NR1 in the assembly of the it a stable the and of the the and in the of of the