Bioscience Biotechnology and Biochemistry · 2006 · 72 citations · 20 references
Life SciencesMicrobiologyMolecular BiologyMicrobial SystematicsMicrobial TaxonomyPhylogeneticsMolecular PhylogeneticsMolecular PhylogenyPhylogenetic AnalysisPhylogenetic ClassificationPhylogenetic Tree ReconstructionTaxonomic ClassificationInternal Transcribed Spacer (Its) ClusteringInternal Transcribed Spacer SequencingMolecular Species IdentificationRibosomal Dna AnalysisRibosomal Dna PhylogeneticsRibosomal Dna SequencingRibosomal Rna Molecular PhylogeneticsRibosomal Rna Phylogeny
In order to establish the molecular phylogeny of the genus Rhizopus, three molecules of the ribosomal RNA-encoding DNA (rDNA), complete 18S, internal transcribed spacer (ITS)1-5.8S-ITS2, and 28S D1/D2 regions of all the species of the genus were sequenced. Phylogenetic trees showed three major clusters corresponding to the three groups in the current morphological taxonomy, microsporus-group, stolonifer-group, and R. oryzae. R. stolonifer var. lyococcos was clustered independently from the major clusters. R. schipperae clustered differently in all trees. Strains of R. sexualis had multiple ITS sequences. A. rouxii clustered with R. oryzae. These results indicate the possibility of molecular identification of species groups using rDNA sequencing. Reclassification of the genus might be appropriate.
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