Molecular‐level pursuit of yeast mitosis by time‐ and space‐resolved Raman spectroscopy

Abstract

Abstract The process of eukaryotic cell division has been studied at the molecular level by Raman spectroscopy. Time‐ and space‐resolved Raman spectra of a living fission yeast cell were successfully recorded with a 100 s time resolution and a 250 nm spatial resolution by using a confocal multi‐channel Raman microspectrometer. Raman spectra characteristic of the nucleus, mitochondrion and septum were identified and time‐dependent changes of major molecular components during the cell cycle were elucidated. Copyright © 2002 John Wiley & Sons, Ltd.