Application of the ΔA method to the determination of morphine

Abstract

Abstract Tablets, suppositories and ear-drops have been assayed using the ΔA method to eliminate interferences. The absorbance at 298 nm is measured for solutions in acid and alkaline media, the difference (Δ A) being taken as a measure of morphine content. The same method has been applied to suppositories after extraction of the drug from the base. Ear-drops containing phenol in addition to morphine were assayed by measuring ΔA at 288 and 298 nm and evaluating the morphine concentration from a pair of simultaneous equations.